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KMID : 0364820190550030313
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Korean Journal of Microbiology
2019 Volume.55 No. 3 p.313 ~ p.315
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Complete genome sequence of Celluosilyticum lentocellum WCF-2 isolated from cow dung
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Heo Jun
Yoo Jae-Hong
Park In-Cheol
Han Byeong-Hak
Kwon Soon-Woo
Ahn Jae-Hyung
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Abstract
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An anaerobic bacterial strain WCF-2 was isolated from cow dung in finding cellulose-degrading bacteria for use as silage additives. Strain WCF-2 showed a higher cellulolytic activity than Cellulosilyticum lentocellum DSM 5427 T , the closest relative of strain WCF-2 (98.2% of 16S rRNA gene sequence similarity).
We sequenced the complete genome of strain WCF-2 and compared it with that of C. lentocellum DSM 5427 T . The OrthoANI value between the two strains was 97.9% thus strain WCF-2 was identified as C. lentocellum. The genome size of strain WCF-2 was 4,779,774 bp with a G + C content of 34.4%, 4,154 coding genes (CDS), 54 pseudo genes, and 142 RNA genes. Strain WCF-2 harbored seven cellulase genes, five of which showed low similarities with those of C. lentocellum DSM 5427 T .
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KEYWORD
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Cellulosilyticum lentocellum, cellulolytic activity, genome sequence
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